Immobilization of 2‐Deoxy‐ <scp>d–</scp> ribose‐5‐phosphate Aldolase on Membrane Supports by Spray‐Coating: Optimization by Design‐of‐Experiment

A strategy for the immobilization of enzyme 2-deoxy-d-ribose-5-phosphate aldolase (DERA) is presented via spray-coating on membrane support material to generate an enzymatically active which can be used in continuously run synthesis modules. functional, water-soluble copolymer containing addressable units covalently bind DERA mixed with enzyme, followed by mixture onto polyacrylonitrile/polyethylene imine- (PAN/PEI) membranes and a subsequent post-processing stabilize coating. Confirmation successful was achieved atomic force microscopy (AFM) imaging assessment retained activity. Through variation relevant parameters, improvements stability activity were seen, formed basis following optimization Design-of-Experiment (DoE). first fractional factorial design yielded additional performance important insights into impacts experimental parameters their interaction. second full did not result further improvements, but validated results design.